Fig. 2 | Nature Communications

Fig. 2

From: Targeting G protein-coupled receptor signaling at the G protein level with a selective nanobody inhibitor

Fig. 2

Kinetic profiling and hydrogen-deuterium exchange (HDX) of the Gβ1γ1-Nb5 complex. Binding of Gβ1γ1 to immobilized Nb5 in SPR equilibrium binding experiments. Association of the Gβ1γ1 dimer with Nb5 was investigated by single-cycle kinetics (a) and affinity based analysis (b). Resonance signals are indicated in response units (RU). The determined dissociation constants (Kd) and kinetic parameters (Kon and Koff) are shown as insets. HDX of the Gβ1γ1 dimer without (c) and with (d) Nb5 is shown with all the identified peptides colored by their percentage of deuterium exchange. e Differential HDX data mapped into the crystal structure of Gβ1γ1 (PDB ID: 5KDO) indicate peptides with increased (greencyan) and reduced (purple) deuterium incorporation upon Nb5 binding (left). Interestingly, Gβ1γ1 peptides that revealed changes in their solvent accessibility during HDX analyses displayed a partial interface with the Gα subunit (right, pink). The Gα subunit was omitted from the left panel for clarity

Back to article page