Fig. 6
From: X-ray structure of a carpet-like antimicrobial defensin–phospholipid membrane disruption complex
Site-directed mutagenesis of NaD1. a The ability of NaD1, NaD1(K36E), and NaD1(R39A) to form multimeric complexes in the presence of PA or PIP2 as determined by protein–protein crosslinking with BS3 and visualized by SDS-PAGE. b Transmission electron micrographs of NaD1, NaD1(K36E), and NaD1(R39A) in the presence of PA or PIP2. While PIP2 binding to all three proteins resulted in fibril formation, PA-mediated fibril formation is abolished for NaD1(R39A). Scale bars represent 200 nm
