Fig. 6

Suppression of PIEZO1 activation by cell surface-inserted LysoPS. a Chemical structures of LysoPS, LysoPA and LysoPC. b–d Suppression of agonist-induced PIEZO1 activation by cell-surface LysoPS. b Schematic model showing suppression of PIEZO1 activation by insertion of LysoPS to the cell surface. c Representative traces (left) and quantification (right) of Yoda1-induced Ca²⁺ influx in WT C2C12 myoblasts treated with vehicle, LysoPS, LysoPA or LysoPC. d Schematic model showing restoration of PIEZO1 activation by removal of cell surface-inserted LysoPS with lipid-free BSA. e Representative traces (left) and quantification (right) of Yoda1-induced Ca²⁺ influx in WT C2C12 myoblasts treated with LysoPS and washed with lipid-free BSA. f, g Impairment of mechanical stimulation-induced PIEZO1 activation by cell-surface LysoPS. f Representative traces of the mechanically-activated current evoked by indentation (8 μm) using a glass probe in PIEZO1-expressing HEK293 cells before (grey, control) and during administration of 5 μM LysoPS (red), followed by removal of LysoPS with lipid-free BSA (blue). g Relative peak currents induced by mechanical stimulation before (control) and during administration of vehicle (grey), 5 μM LysoPS (red), and 5 μM LysoPC (blue) in f. *P < 0.05 and ****P < 0.0001 (Student’s t-test). NS not significant, n sample number. Bar graphs represent mean ± S.E.M. Box and whiskers graph―line: median, box: upper and lower quartiles, whiskers: maxima and minima