Fig. 6
From: Male germ cells support long-term propagation of Zika virus
ZIKV infects human male GCs and BC reduces ZIKV infection. a, b Mock-infected and ZIKV-infected (IFU = 1 × 107) human testis immunostaining with DDX4 and ZIKV-E (a) and quantification (n = 4 for mock-infected and 7 for ZIKV-infected) (b) at 72 hpi. c–e qRT-PCR analysis of ZIKV cellular vRNA in human testis (n = 4 for mock- and 8 for ZIKV-infected) (c), ZIKV negative-strand vRNA in human testis (n = 6 for mock-infected and ZIKV-infected) (d), and ZIKV vRNA in human testis culture supernatant (n = 5 for mock-infected and 4 for ZIKV-infected) (e) of mock-infected and ZIKV-infected (IFU = 1 × 107) human testis samples at 72 hpi. f Infectivity of supernatant from mock-infected and ZIKV-infected human testis as assessed with plaque assay using Huh-7.5 cells (n = 4 for mock-infected and 6 for ZIKV-infected). g–i qRT-PCR analysis of ZIKV cellular vRNA in human testis (g), ZIKV negative-strand vRNA in human testis (h), and ZIKV vRNA in human testis culture supernatant (i) of BC-treated and DMSO-treated ZIKV-infected human testis samples. j Infectivity of supernatant from BC-treated and DMSO-treated ZIKV-infected human testis as assessed with plaque assay using Huh−7.5 cells (n = 4 for DMSO and BC for 48 h and n = 6 for DMSO and BC for 72 h). Statistical values are presented as mean ± s.e.m. and analyzed by two-sided t test, *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001. Scale bar, 50 μm. ZIKV strain is MR 766 throughout
