Fig. 3
From: Synthetic cytokine receptors transmit biological signals using artificial ligands
Microarray analysis of Ba/F3-SyCyR(IL-23/2 A) cells. a Venn-diagram of at least 1.5-fold (p-value ≤ 0.01) up- or downregulated mRNAs of Ba/F3-SyCyR(IL-23/2A) cells stimulated with 100 ng/ml GFP-mCherry (light gray) and Ba/F3-IL-12Rβ1-IL-23R cells stimulated with 100 ng/ml HIL-23 (dark gray) for 60 min. Microarray analysis was performed using samples of four independent biological replicates. b Heat map comparing mRNA levels of Ba/F3-SyCyR(IL-23/2A) and Ba/F3-IL-12Rβ1-IL-23R stimulated and unstimulated as described in a. c Higher magnification of selected mRNAs from b. d Ingenuity pathway analysis (IPA) revealed the top five canonical pathways of Ba/F3-SyCyR(IL-23/2A) vs. Ba/F3-IL-12Rβ1-IL-23R
