Fig. 3

Eed and Suz12 are required for the stability of Ezh2 on chromatin, but have different effects on its target search process. a Schematic representation of Ezh2. The N-terminus of Ezh2 interacts with Eed. The SANT2 domain (red rectangle) interacts with Suz12. The C-terminus of Ezh2 is the catalytic lobe, including cysteine-rich domain (CXC, yellow rectangle) and SET domain (green rectangle) that is the catalytic domain of Ezh2. b Cumulative distribution of displacements for HaloTag-Ezh2 replicated from Fig. 1, for HaloTag-Ezh2 (N = 64 cells, n = 11437 displacements) in Eed^−/− mES cells, and for HaloTag-Ezh2^ΔSANT2(N = 71 cells, n = 5981 displacements) in wild-type mES cells. The cumulative distributions were fitted with three populations. Fitted parameters are shown in Supplementary Table 3. c Fraction of the chromatin-bound population (F₁) obtained from Fig. 3b. Results are means ± SD. d Survival probability distribution of the dwell times for HaloTag-Ezh2 replicated from Fig. 2, for HaloTag-Ezh2 (N = 93 cells, n = 2339 trajectories) in Eed^−/− mES cells, and for HaloTag-Ezh2^ΔSANT2 (N = 85 cells, n = 2728 trajectories) in wild-type mES cells. The distributions were fitted with a two-component exponential decay model. Fitted parameters are shown in Supplementary Table 4. e–g Fraction (e), residence time (f), and search time (g) of the long-lived population (F_1sb) for HaloTag-Ezh2 replicated from Fig. 2, for HaloTag-Ezh2 in Eed^−/− mES cells, and for HaloTag-Ezh2^ΔSANT2 in wild-type mES cells. Results are means ± SD from three biological replicates