Fig. 6

In utero electroporation of GFP and SETBP1-G870S. a Schematic representation of the electroporation procedure. b Snapshots from 3D reconstruction resulting from 2-photon microscopy on either GFP- or SETBP1-electroporated cortices (2 days) after tissue clarification (X-Clarity system) showed defects in radial migration of the SETBP1-G870S misexpressing cells. p pial side, v ventricular side. The GFP signal in the pial membrane (white arrows) and along the thickness of the SETBP1-G870S tissue (red arrow) is due to the basal processes of the GFP+ radial glia cells located in the deepest part of the organ. c Immunohistochemistry for GFP and TBR2 on coronal section of 2 days electroporated tissues. d Five-day electroporated cortices and quantification of the migration of the GFP+ cells from apical (bin #1) to pial part of the organ (bin #5); arrow indicates GFP+ corpus callosum. Statistical analysis was performed using two-way ANOVA; error bars represent standard error. *p < 0.05; **p < 0.01; ***p < 0.001; ***p<0.0001.  e Immunohistochemistry for GFP and SATB2 marker on coronal section of 5 days electroporated tissues. In the insets on the right, the images of SETBP1-G870S DAPI (up), GFP, SATB2, and merge of GFP/SATB2 (bottom) are shown. cp cortical plate, iz intermediate zone, svz subventricular zone, vz ventricular zone. Bars: c, e: 100 μm, d: 250 μm