Fig. 3 | Nature Communications

Fig. 3

From: Reliance upon ancestral mutations is maintained in colorectal cancers that heterogeneously evolve during targeted therapies

Fig. 3

Functional restoration of WTAPC induces cell death in CRC cells carrying distinct mechanisms of secondary resistance. a–c Parental and resistant-derivatives CRC cells were electroporated with plasmid encoding for WTAPC or an inactive APC version (G97*). Electroporation buffer alone was used as control (mock). After 48 h, cells were stained with Hoechst 3342 /Propidium Iodide (PI) to detect cell death. Representative images of single 96-wells are shown for each condition. d–f Relative quantification of Hoechst/PI positive cells was made using ImageJ software and normalised against mock cells. Results represent means ± SD of three independent wells

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