Fig. 6 | Nature Communications

Fig. 6

From: Reliance upon ancestral mutations is maintained in colorectal cancers that heterogeneously evolve during targeted therapies

Fig. 6

Cell death associated with porcupine blockade is independent from MAPK alterations. MAPK-resistant CRC cells were treated with 1 µM LGK974 (WNTi) for 2 weeks. After that, gDNA was extracted from the control untreated (NT) and the LKG974-treated cells. ddPCR analysis was performed to measure the fractional abundance of the mutated alleles, previously identified as mechanisms of secondary resistance to MAPK inhibition. Results represent means ± SD of two independent technical replicates. a and b indicate the independent biological replicates of the experiment

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