Fig. 4
From: Molecular principles underlying dual RNA specificity in the Drosophila SNF protein
dSNF1–101-RNA NMR chemical shift perturbations. a Backbone amide chemical shift differences between dSNF1–101 and dSNF1–101–dU1SLII complex. Δδ = [(ΔδHN)2 + ((ΔδN)2*0.154)]1/2. dSNF1–101 at 300 µM, dU1SLII at 700 µM. b dSNF1–101 backbone amide chemical shift perturbations (Δδ) upon dU2SLIV binding. dSNF1–101 at 300 µM, dU2SLIV at 1.1 mM. c Difference plot of chemical shift perturbations (Δ(Δδ) = [Δδ(dSNF1–101–dU2SLIV) – Δδ(dSNF1–101–dU1SLII)]). Positions of secondary structure elements are shown on the top. 50 mM KCl, 20 mM sodium cacodylate, pH 6.5, in 90% 1H2O, 10% 2H2O; 23 °C; 700 MHz. d Orthogonal views of Δδ (top and middle) or Δ(Δδ) values (bottom) mapped onto the structure of dSNF1–96. A scale bar is shown on the top. The orientation of the left panels relative to Fig. 1b is shown at the bottom
