Fig. 7
From: Macrophage migration inhibitory factor is required for NLRP3 inflammasome activation
MIF co-localizes with the NLRP3 inflammasome. a ASC-cerulean macrophages were primed with LPS (10 ng ml−1) overnight. The following day cells were treated with nigericin (10 µM) for the indicated times. Co-localization of ASC (green) and MIF (red) were visualized using confocal microscopy. Confocal images are representative of at least three independent experiments. b Primary murine BMDMs were treated with LPS (10 ng ml−1) overnight, and then treated with or without COR123625 (50 µM), followed by nigericin (5 µM) for 20 min. Cells were fixed and stained with antibodies against NLRP3 and MIF. Nuclei were stained with DAPI. All scale bars = 10 μm
