Fig. 2
Peptide-appended pillar[5]arene (PAP) channels maintain an aquaporin-like single channel permeability in block copolymer bilayers. a Confocal fluorescence microscope image of a giant unilamellar vesicle showing the incorporation of fluorescently labeled PAP channels into PB23 polymersomes. Block copolymers were not labeled. Scale bar, 5 μm. b Representative light scattering traces of PB23 polymersomes with different molar channel-to-polymer ratios (mCPRs) after a rapid exposure to a hypotonic solution in a stopped-flow instrument. The hypotonic solution lacked the 100 mM PEG600 used to form the vesicles. c The water permeability of PAP channel-containing PB23 polymersomes formed with different mCPRs measured under hypotonic conditions. The dash line showed the background permeability level of a control PB23 vesicle. d Net water permeability of PAP channels in liposomes (4:1 (mol/mol) phosphatidylcholine/phosphatidylserine (PC/PS), molar channel-to-lipid ratio (mCLR) is 0.005), and in PB23 polymersomes (mCPR = 0.005) after subtraction of the background permeability of control vesicles shown in c. e Single channel water permeability of PAP channels in PC/PS liposomes and PB23 polymersomes. The permeabilities of AQP1, AQP0 and CNTP are from Zeidel et al.30, Saobe et al.31, and Tunuguntla et al.12, respectively. f The insertion efficiency of PAP channels in PC/PS liposomes and PB-PEO polymersomes was determined using fluorescence correlation spectroscopy (FCS) measurements. The calculation of insertion efficiency is described in detail in Supplementary Methods. Data shown are the average of triplicates with standard deviation
