Fig. 3

HuR associates with hTERT in a TERC-dependent manner. a U2OS cells were co-transfected with a vector expressing flag-hTERT together with a vector expressing TERC for 48 h. IP assays were performed to assess the association of flag-hTERT and HuR. Data are representative from three independent experiments. b HeLa cells were transfected using a vector that expressed flag-hTERT. Forty-eight hours later, lysates were prepared and subjected to IP assays by using antibodies indicated. The IP materials were used to test the telomerase activity by using TRAP assays. Data are the means ± SD from three independent experiments