Fig. 7

HuR-telomerase axis impacts on the renewal of mHSCs. a Twelve weeks after transplanting, GFP⁺ donor-derived LT-HSCs (Lin⁻Sca1⁺cKit⁺FLT3⁺CD34⁻) were isolated from primary recipients of TERC^+/− group or G3TERC^−/− group (n = 5) and subjected to single-cell qPCR analysis to determine the telomere length. The ΔCT values of the qPCR data are represented as the means ± SD; significance was analyzed by using Student’s t-test (**p < 0.01). b, c Contribution of the GFP⁺CD45.2⁺ (with silenced HuR) cells to the indicated PB (b) and LT-HSC populations (c) at 12 weeks after transplantation, respectively. Data represent the means ± SD from five mice; significance was analyzed by using Student’s t-test (**p < 0.01). d–f G3TERC^−/− mHSC (LSK) cells were infected with lentiviruses expressing GFP together with TERC or its variants (U15A + U58A or C64G). The sorted cells were further treated and analyzed same as described in (a–c)