Fig. 2
Mechanisms 1 and 2 control biofilm formation and the expression levels of RsmY, while all three mechanisms contribute to controlling RsmZ levels. a Quantification of biofilm formation on peg-lidded 96-well plate by crystal violet staining. Plates inoculated with the mutant strains were incubated for 10 h with shaking at 37 °C. Error bars show SEM (three biological repeats each containing five technical repeats). *Indicates significantly different comparisons (P < 0.05, one-way ANOVA). See also Supplementary Fig. 4 for these mutations in a ΔgacS background. b, c Relative expression level of RsmY (b) and RsmZ (c) in the mutant strains relative to the wild-type PAO1 strain. RNA levels were measured using qRT-PCR. Error bars show SEM (three biological repeats, with three technical repeats per biological repeat). *Indicates significantly different comparisons (P < 0.05, one-way ANOVA). The mechanisms disabled in each mutant strain are indicated
