Fig. 2
From: Nicotine aversion is mediated by GABAergic interpeduncular nucleus inputs to laterodorsal tegmentum
IPN inputs to LDTg are GABAergic and mediate aversion. a Left: schematic of viral and dye injections. Right: schematic of recording set-up. b Representative light-evoked synaptic currents before and during bicuculline (100 ms, 20 pA scale). c Normalized light-evoked synaptic current amplitudes before, during, and after bicuculline application (20 μM); one-way RM ANOVA, F1.147, 6.882 = 7.863, P = 0.0246; Tukey: P = 0.0188, P = 0.0321, n = 7 cells from 5 mice. d Current–voltage relationship, (grey circle) ECl = −125 mV, (white circle) : ECl = −17 mV. (linear regression, 95%CI: −144.6 to −78.51; 95%CI: −23.78 to 16.94; slopes: P = 0.4098; reversals: −98.30 mV and −6.671 mV, adjusted for junction potential: −114.2 mV and −16mV; n = 2 cells per condition from 2 mice). e Behavioral experiment timeline and schematic. f RTPT preference scores for 20 Hz stimulation; unpaired t-test (one-tailed), t19 = 1.774, P = 0.0460, n = 12, n = 9 mice per group. g Representative heat maps of animal positions (20 Hz and 1 Hz stimulation). h Time spent on photo-stimulation side (blue box) and no-stimulation side (grey box) with 20 Hz stimulation. i Same as h but with 1 Hz stimulation. Data presented as mean ± SEM, * P< 0.05
