Fig. 7
From: Molecular basis for the production of cyclic peptides by plant asparaginyl endopeptidases
The MLA as a diagnostic marker for the discovery of ligase-type AEPs. a Sequence alignment of H. enneaspermus AEPs (see also Supplementary Fig. 11). HeAEP3 was predicted to be a ligase due to the truncation within the MLA and the presence of a unique Gatekeeper residue. b, c MALDI-MS analysis of N. benthamiana-produced peptides upon co-expression of (b) HeAEP1, 2 and 3 with Oak1 and (c) GrAEP1 with Oak1. For all treatments, means and s.e.m. are of the percentage MS signal obtained for cyclic kB1 over the total MS signal of all kB1-related peptides produced. Treatments carrying unique Greek lettering are significantly different (P < 0.05) as determined by Tukey’s ANOVA. Replicates are as follows: n = 8 (no AEP, HeAEP1, HeAEP2, HeAEP3) and n = 6 (GrAEP1). Error bars are s.e.m.
