Fig. 1
SAMHD1 (de)phosphorylation at T592 is regulated in a cell cycle dependent manner. a, b HeLa cells were arrested at the G1/S border using a double-thymidine block. After the 2nd release, synchronized cells were harvested at different time points post-release (p.r.). Respective samples were split for immunoblotting (a) and propidium iodide (PI) staining (b) to determine cell cycle-phases by flow cytometry. For immunoblotting, whole-cell lysates were analyzed using antibodies specific to the indicated proteins. Data shown are representative of two independent experiments. c HeLa cells were arrested at the G1/S border and harvested at different time points as described in Fig. 1a. Respective samples were split for dATP measurement, immunoblotting (Supplementary Fig. 1a, c) and PI staining (Supplementary Fig. 1b). dATP was quantified by single nucleotide incorporation assay and normalized by protein content of the corresponding lysate. Data shown represent the mean ± SD of two independent experiments
