Fig. 4

RIM1 KD in the CA1 region impairs hippocampus-dependent memory. a Experimental designs for the object location task. b In the acquisition phase of the object location task, RIM1 KD and control mice spend similar time exploring the two objects; n = 10 mice/group, t-test, p > 0.05. c In the test phase of the object location task, RIM1 KD mice fail to show preference for the relocated object; n = 10 mice/group, t-test, *p < 0.05. d Experimental design for the temporal order memory task. e In the temporal order memory task, control mice prefer the object explored early to that explored last, whereas mice with RIM1 KD have impaired performance in this task; n = 10 mice/group, t-test, *p < 0.05. f Experimental design for the novel object preference task. In both the acquisition phase (g) and the test phase (h) of the novel object preference task, RIM1 KD and control mice spend similar time exploring the two objects; n = 10 mice/group, t-test, p = 0.34. i In the open field test, RIM1 KD and control mice show similar locomotor activity during 15 min of open field exploration; n = 10 mice/group, t-test, p > 0.05. j RIM1 KD mice and control mice spend similar time in the center of the open area during the first 5 min of exploration; n = 10 mice/group, t-test, p > 0.05. k In contextual fear conditioning, there is no significant difference between RIM1 KD and control mice in freezing responses during the last 2 min of training; n = 10 mice/group, t-test, p > 0.05. l Twenty-four hours after conditioning, RIM1 KD mice show a significant reduction in the time spent freezing; n = 10 mice/group. Bar graphs and individual points represent the mean ± s.e.m.; t-test, *p < 0.05