Fig. 5
From: Cyclophilin A enables specific HIV-1 Tat palmitoylation and accumulation in uninfected cells
Tat palmitoylation inhibits its secretion. a Jurkat cells were transfected with Tat or Tat-C31S before treatment for 3 h with 50 µM 2-BP or palmitate as indicated and assaying Tat secretion by ELISA (Mean ± SEM, n = 3 independent experiments performed in triplicate). b PC12 cells were treated similarly before assaying Tat secretion using immunoprecipitation and Western blotting. Results from a representative experiment are shown together with the quantification (Mean ± SEM, n = 3). The significance of differences with controls was assessed using one-way ANOVA (***p < 0.001; **p < 0.01; *p < 0.05)
