Fig. 2
Aptamer-mediated targeted delivery of large and small RNAs. Cell-targeting aptamers, Waz and C10.36, and their respective controls were assembled with 3WJdB (a) or AF488-anti-tail (b), and their ability to bind the same batch of NALM6 cells was assessed after 1 h incubation by flow cytometry. Similarly, Waz and C10.36, and their respective controls were annealed with AF488-labeled 3WJdB (c) or AF488-anti-tail (d), and their targeting properties were assessed using the same batch of NALM6 cells. Representative flow cytometry curves illustrate a shift in fluorescence for aptamer nanostructures bearing both Waz (blue) and C10.36 (magenta) assembled with the RNA payloads. Cells treated only with DFHBI-1T (20 μM) are shown in green. All non-targeted controls are shown in orange: free 3WJdB in a, control aptamer assembled with AF488-anti-tail in b,d, free 3WJdB-AF488 in c. Gray filled curves: unstained cells. Normalized cell counts are reported on the y-axis, while on the x-axis is shown a log scale of fluorescence intensity. Geometric mean fluorescence intensity of Waz (blue), C10.36 (magenta), and non-targeted controls (orange) are shown above the respective curves in c, d. All curves in c, d are representative of two independent experiments. Geometric mean fluorescence intensity values for leukemia cell lines are reported in e using 3WJdB stained with DFHBI-1T as payload and in f using AF488-anti-tail as payload. Representative flow cytometry curves for MEC1, MEC2, MOTN1, and SP1 cell lines are shown in Supplementary Fig. 6. Values are the mean ± SD for at least three independent experiments. Statistical analysis for comparing multiple groups in each cell line was analyzed by one-way ANOVA with post hoc Tukey’s test. Brackets with asterisks represent statistical difference: ns not significant; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001
