Fig. 6

Eg5 limits retrograde microtubule growth in dendrites by microtubule stabilization. a Kymographs revealing EB3 comet dynamics in distal dendrites of DMSO-, STLC-, or FCPT-treated neurons. Neurons were treated with the drugs at 3 DIV, transfected with the reporter EB3-tomato at 8 DIV, and analyzed by time-lapse microscopy at 9 DIV. White arrowheads indicate retrograde EB3 comets. b Quantification of EB3 comet polarity in proximal and distal segments of dendrites in neurons as in (a); n = 3 to 6 independent experiments. Columns show means and dot overlays individual data points. Total of 29 (DMSO), 29 (STLC), and 30 (FCPT) dendrites. *P < 0.05, ***P < 0.001 by two-tailed t-test. Error bars: s.e.m. c Neurons treated as in (a) were fixed and stained with antibodies against α-tubulin and acetylated α-tubulin as indicated. Scale bar, 25 μm. d Quantification of α-tubulin (green dots) and acetylated α-tubulin (red dots) staining intensity in proximal and distal dendrite regions of neurons as in (c). Each dot represents the average value for one cell (2–4 dendrites); n = 43 neurons (DMSO), 29 (STLC), and 20 (FCPT) from 3 independent experiments. Black horizontal line: mean of the three experiments. *P < 0.05, ****P < 0.0001, by two-tailed t-test