Fig. 7
From: NEK7 regulates dendrite morphogenesis in neurons via Eg5-dependent microtubule stabilization
A fraction of EOS-tagged Eg5 in dendrites is stably bound to microtubules. a Time-lapse image series showing photoconverted EOS signal in dendrites of 9 DIV neurons expressing EOS, EOS-tagged Eg5, or EOS-tagged α-tubulin. Blue boxes indicate the photoconverted region at time point 0. b Increase and decay of the photoconverted EOS signal intensity over time in the targeted area of dendrites as in (a). For each sample signal intensities were normalized to the intensity at the time point of photoconversion, which was set to 1. For each time point mean values from three different experiment were plotted; n = 3 experiments, total of 9–19 neurons per condition. Error bars: s.e.m. c Fluorescence intensity distributions of photoconverted EOS at four different time points along photoconverted and adjacent regions in dendrites shown in (a). Blue shading delimits the section that was photoconverted
