Fig. 1

Hem-1^−/− mice exhibit decreased HPCs and HSCs, and die prematurely. a Genetic characterization of Hem-1^+/− offspring, demonstrating that Hem-1^−/− mice are born with appropriate Mendelian frequency. b Hem-1^−/− mice have growth retardation compared to littermate Hem-1^+/+ mice (Hem-1^+/+ mice, n = 6; Hem-1^−/− mice, n = 8, **p < 0.01, Student’s t test). c Hem-1^−/− mice die an average of 6 weeks after birth (n = 13, p < 0.001, log-rank test). d Flow cytometric analytic schematic of hematopoietic stem and progenitor cells in Hem-1^−/− and littermate Hem-1^+/+ mice. (FSC: forward scattered light, Lin⁻: CD3e⁻/CD11b⁻/CD45R⁻/B220⁻/Ter-119⁻/Gr-1⁻, LSK: Lin⁻/Sca-1⁺/c-Kit⁺, HPC: Lin⁻/Sca-1⁻/c-Kit⁺, HSC: LSK/CD150⁺/CD48⁻). e E14.5 fetal liver hematopoietic stem and progenitor cells subsets are not different between Hem-1^−/− and littermate Hem-1^+/+ mice (n = 10). f E14.5 FL cobblestone area-forming cells (CAFCs) are not different between Hem-1^−/− and Hem-1^+/+ mice (n = 3). g Five-week Hem-1^−/− BM exhibits decreased hematopoietic stem and progenitor cell subsets (n = 5, **p < 0.01, ***p < 0.001, Student’s t test). h Five-week Hem-1^−/− BM CAFCs are reduced compared to littermate Hem-1^+/+ mice (n = 3, *p < 0.05, **p < 0.01, Poisson statistics). Error bars represent the mean ± SD