Fig. 2
From: The Wave2 scaffold Hem-1 is required for transition of fetal liver hematopoiesis to bone marrow
Hem-1 deletion leads to an intrinsic functional defect in HSCs. a Schematic of rescue FLC transplantation where Hem-1−/− CD45.2 HSCs failed to rescue lethally irradiated wild-type CD45.1 recipient mice (n = 8, p < 0.001, log-rank test). b E14.5 Hem-1−/− CD45.2 FL HSCs could not compete with recipient-derived CD45.1 BM cells (BMC) in repopulating lethally irradiated Hem-1+/+ adult recipient mice. Blood was analyzed monthly after transplantation and marrow at 4 months post transplantation (n = 5, ***p < 0.001, Student’s t test). c Schematic of the competitive repopulation assay where exogenous littermate Hem-1+/+ CD45.1 HSCs efficiently rehabilitated the hematopoietic system in Hem-1−/− CD45.2 mice via a reverse stem cell transplantation assay in non-ablated mice. BMCs, HPCs, and HSCs were analyzed at 4 months post transplantation (n = 8, ***p < 0.001, Student's t test). d Littermate Hem-1+/+ BM HSC rescued growth retardation and premature death when transplanted into non-ablated Hem-1−/− mice (n = 12, *p < 0.05, ***p < 0.001, body weight: two-way ANOVA; percentage survival: log-rank test)
