Fig. 4

Hem-1 deletion results in a progressive decrease of HSCs mediated by increased apoptosis. a Hem-1 deletion leads to the loss of HPCs and HSCs in a developmentally dependent manner. BM cells were harvested at E18.5, postnatal day (PD) 1, 3, and 7, and total BM cells (BMCs), HPCs, and HSCs were determined (n = 5, *p < 0.05, ***p < 0.001, one-way ANOVA). b HPCs and HSCs from Hem-1^−/− mice showed no significant difference in cell cycle distribution except PD7 compared to the cells from littermate controls (n = 5, **p < 0.01, ***p < 0.001, χ² test). c Hem-1^−/− HPCs and HSCs had progressive increases in the fraction of apoptosis as measured by Annexin V staining compared to littermate controls (n = 5, **p < 0.01, ***p < 0.001, Student's t test)