Fig. 5
Altered macrophage markers in rheumatoid arthritis in Mmp12–/– mice. a Hind ankle widths of Mmp12+/+ and Mmp12–/– male B10.RIII mice (n = 18 and 20, respectively, for each time point) after onset of collagen-induced arthritis (day 0), means ± s.e.m. Mann–Whitney t-test: *p < 0.05, **p < 0.01, ***p < 0.005. b Histopathology of hind ankles after H&E staining (day 18) Mmp12+/+ (n = 3) and Mmp12–/– (n = 3). Bone destruction (p < 6 × 10−5), pannus formation (NS not significant), synovial hyperplasia (p < 3 × 10−5), and subsynovial inflammation (p < 7 × 10−4) were quantified as a histopathological score (p < 9 × 10−4, means ± s.d.), two-tailed unpaired Student’s t-test: *p < 0.05, **p < 0.01. c Representative images of H&E, IFN-γ, iNOS, MHCII, and CD36 immunostaining of hind ankle joints of Mmp12+/+ (n = 3) and Mmp12–/– (n = 3) mice. Here and in g: N NETs; C cartilage; S synovial space; arrowheads, high antibody or H&E staining; scale bars, 100 μm. d Immunostaining quantification of Mmp12+/+ versus Mmp12–/– male B10.RIII mice hind ankle joints for IFN-γ (p < 2 × 10−5), iNOS (p < 1 × 10−3), MHCII (p < 2 × 10−5), and CD36 (p < 2 × 10−2) (means ± s.d.), two-tailed unpaired Student’s t-test: *p < 0.05, **p < 0.01, ***p < 0.005. e Ninety-day-female MRL/lpr mice were injected with CFA (day 0). Hind ankle size was measured in Mmp12+/+and Mmp12–/– MRL/lpr mice (n = 30 and 28, respectively, for each time point) (means ± s.d.), two-tailed unpaired Student’s t-test: ***p < 0.005. f Histopathology of H&E stained-hind ankle joints of Mmp12+/+ (n = 3) and Mmp12–/– (n = 3) MRL/lpr (day 25). Bone destruction (n.s.), pannus formation (p < 0.01), synovial hyperplasia (p < 3 × 10−2), and subsynovial inflammation (p < 3 × 10−2) were quantified as the histopathological score (p < 6 × 10−3) (means ± s.d.), two-tailed unpaired Student’s t-test: *p < 0.05, **p < 0.01. g Representative images of H&E, IFN-γ, iNOS, MHCII, and CD36 immunostaining of hind ankle joints of Mmp12+/+ and Mmp12–/– MRL/lpr female mice. BM bone marrow. h Immunostaining intensities of Mmp12+/+ and Mmp12–/– mouse hind ankle joints for IFN-γ (p < 2 × 10−5), iNOS (p < 1 × 10−3), MHCII (p < 4 × 10−4), and CD36 (p < 4 × 10−4) (means ± s.d.), two-tailed unpaired Student’s t-test: ***p < 0.005
