Fig. 7
IFN-γ epitope antibody staining of human lupus nephritis biopsies. a Left, amino acid sequences (underlined) of human IFN-γ peptides used to raise and affinity-purify rabbit anti–N-terminal, C-terminal-1, and C-terminal-2 IFN-γ epitope antibodies. Right, western blot analysis of human IFN-γ after time-dependent cleavage to 1080 min by human MMP12. Note: disappearance of the C-terminal epitopes as MMP12 cleavage proceeds. Molecular weight marker positions in all blots are as shown. b Immunostaining of human kidney biopsies using anti–N-terminal, C-terminal-2, C-terminal-1, and MMP12 antibodies; and staining with hematoxylin and eosin (H&E), Trichrome, Jones, and PAS of kidney biopsies of healthy (n = 5), lupus nephritis at Grades III-(A) (n = 3) and IV-(A) (n = 2) as diagnosed in Supplementary Fig. 11. Scale bar, 100 μm. Original magnification, ×400. c Quantification of immunostaining intensities in healthy (n = 5) and lupus nephritis (n = 5) kidney biopsies (additional data are included in Supplementary Fig. 11) and expressed as means ± s.d. Statistical significance was determined by a two-tailed unpaired Student’s t-test: ***p < 0.005
