Fig. 3

Elevated cell adhesion molecule expression on SECs. a qPCR analysis of adhesion molecules, Selp, Sele, Icam1, and Vcam1 of sorted AECs and SECs. Data are represented as mean ± SEM. n = 4 for AECs and SECs. Data were analysed with two-tailed t-test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. b PDPN can be substituted for antibody against the adhesion molecule ICAM1 and E-selectin. Shown is EC isolation from a wild-type mouse injected i.v. with anti-PDPN and the enzymatically dissociated bone marrow cells were stained with other antibodies ex vivo and pre-gated on singlet, live, CD45⁻, Ter119⁻, CD31⁺ cells. c Anti-CD31 is dispensable for the analysis of bone marrow AECs and SECs. Flushed enzymatically dissociated bone marrow cells were stained with anti-CD45, anti-Ter119, anti-ICAM-1/anti-CD62E, anti-CD31, and anti-Sca-1 ex vivo. Analysis was pre-gated on singlet, live, CD45⁻, Ter119⁻ cells. d P-selectin marks sinusoids. Anti-P-selectin and anti-CD31/CD144 were i.v. injected into Nestin-GFP mice and femur was imaged after removal. Scale bar, 50 um. e ICAM-1 marks sinusoids. Anti-ICAM-1 and anti-CD31/CD144 were i.v. injected into Nestin-GFP mice and femur was imaged after harvest. Scale bar, 100 um. f E-selectin marks sinusoids. Anti-E-selectin, anti-Sca-1, and anti-CD31/CD144 were i.v. injected into wild-type mice and femur was imaged after harvest. Scale bar, 50 um