Fig. 3
From: Efficient generation of mouse models of human diseases via ABE- and BE-mediated base editing
The generation of Hoxd13 and Tyr mutant mouse models by SaBE3 and SpABE. a Fused digits and white hair phenotypes in founder mice. Left: Mutant mouse shows whole-body white hair and fused digits (purple arrowhead). Right: all the four mice displayed whole-body white hair and fused digits. b Representative alignments of modified sequences from newborn pups after microinjection of SpABE/SaBE3 mRNA and sgRNAs targeting Hoxd13 and Tyr simultaneously. The PAM sequences and substitutions are highlighted in blue and red, respectively; N/N indicates positive colonies out of the total sequenced. The corresponding targeted codons are shown. c Summary of phenotypes. Three mice displayed normal hair and fused digits; nine mice displayed black-and-white hair and fused digits; four mice displayed whole-body white hair and fused digits; nine mice displayed normal hair and normal digits; one mouse displayed black-and-white hair and normal digits; no mice displayed whole-body white hair and normal digits. d Representative alignments of modified sequences from newborn pups harboring indel. The PAM sequences and substitutions are highlighted in blue and red, respectively; N/N indicates positive colonies out of the total sequenced. The corresponding targeted codons are shown. e Summary of the genotyping of Hoxd13 and Tyr mutations of newborns by SaBE3 and SpABE-mediated base editing
