Fig. 6 | Nature Communications

Fig. 6

From: The TRPV4 channel links calcium influx to DDX3X activity and viral infectivity

Fig. 6

TRPV4 acts as a host factor for viral replication. a Mean (±SEM) of normalized intracellular Ca2+ obtained from 3 experiments (with 70.000 cells analyzed in each experiment). Huh7 cells were loaded with the calcium indicator Calbryte 520 AM and exposed to control, ZIKV or ZIKV+10 μM HC067047. b Mean ( ± SEM) of normalized intracellular Ca2+ signals (Fura-2 ratio) obtained in Huh7 cells exposed to control (n = 38), ZIKV (n = 48) or ZIKV+10 μM HC067047 (n = 37) conditions. c Confocal immunofluorescence images of DDX3X in Huh7 cells exposed to ZIKV in the presence or absence of HC067047. Nuclei were stained with DAPI (blue). Colocalization of DDX3X and nuclei is shown in white in the merge panels. d Mean nuclear/total DDX3X ratio determined under the experimental conditions indicated. e DENV, HCV and ZIKV infection in Huh7 cells treated with increasing concentrations of HC067047. The data are presented as Mean (±SEM) of three independent experiments at each HC067047 concentration. f DENV infection in Trpv4+/+ and Trpv4−/− MEFs. Cellular viability was measured by CytoTox-Glo cytotoxicity assay and viral infectivity was measured as luciferase activity. The data are presented as Mean ( ± SEM) of three independent experiments. Data are means ± SEM. **P < 0.01 when comparing WT with any other condition as determined by one-way ANOVA followed by a Bonferroni post hoc test (d) or when comparing Trpv4+/+ and Trpv4−/− mice as determined by two-tailed Student’s t test (f). P = 0.001 for infectivity and P = 0.2 for cell viability in f. Scale bar = 10 μm

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