Fig. 2
Full-field ERG recording. a Schematic of ffERG recording with ganzfeld stimulation on cynomolgus monkeys. b, c Photograph of a GRACE device and Jet electrode applied to an eye of a cynomolgus monkey, respectively. Scale bar, 5 mm. d–i Representative ffERG signals recorded with a GRACE device (red) and a Jet electrode (blue) from the same eye of a cynomolgus monkey, following the guidelines set by the ISCEV. The solid lines show the average signal and the shaded regions show standard deviation. n = 3 from same eye. d, e Scotopic (dark-adapted) ERG responses under 0.01 and 3.0 cd s m−2. f Scotopic oscillatory potentials (OPs) recorded under 3.0 cd s m−2. g Scotopic ERG responses under 10.0 cd s m−2. h Photopic (light-adapted) ERG responses under 3.0 cd s m−2. i Photopic 30 Hz flicker ERG responses under 3.0 cd s m−2. Different categories are presented here according to the order of recording. ‘a’ and ‘b’ mark the cornea-negative a-wave and the cornea-positive b-wave, and N1, N2, N3, P1, P2, P3…label the wavelets in oscillatory potentials. The dashed lines in i mark the midpoints of the stimulus flashes. Note that the a-wave for scotopic ERG under 0.01 cd s m−2 is absent because of the weak stimulus. j, k Summary of the implicit times and amplitudes of various ERG responses recorded by the GRACE and Jet electrodes on cynomolgus monkeys. Scot. and Phot. are the abbreviations for scotopic and photopic, respectively. The number marks the stimulus intensity. The measurements of implicit times and amplitudes for characteristic waves in various ERG responses can be found in Methods section and Supplementary Figure 2. Inset of k, RMS noise level comparison between GRACE and Jet electrode recordings. The bottom and top of the box are the first and third quartiles, the band and dot inside the box is the second quartile (the median) and mean value, respectively, the ends of the whiskers represent the minimum and maximum of all of the data. ***p ≤ 0.001, **p ≤ 0.01, *p ≤ 0.05, NS, p > 0.05; n = 30 from 10 eyes, Bonferroni correction for p-value, Wilcoxon signed-rank test analysis
