Fig. 3
From: The 3.3 Å structure of a plant geminivirus using cryo-EM
In vivo assembly assay for genome encapsidation. Representative negative stain micrographs of particles produced by infiltration with AYVV DNA-A (a) or co-infiltration with AYVV CP and DNA−α (b). Relative composition of “geminate” particles (pink in bar chart) compared with “single” particles (green in bar chart) from AYVV (c), and co-infiltrations of AYVV CP, R48A, and M59D, each with DNA−α (c). Representative negative stain micrographs and 2D class averages are shown for R48A variant (d) and M59D variant (e). The yellow asterisk highlights 2D class averages which appear to be “single” VLPs with a missing pentameric capsomer. Error bar is 100 nm
