Fig. 6

Cross-talk between PRMT5-dependent histone arginine methylation and lysine acetylation. a Representative experiment of sequential PRMT5-mediated methylation of arginine residues on histone H4 followed by KAT-dependent acetylation of histone H4 by KAT7 (lanes 1–3), KAT5 (lanes 4–6), and KAT2A (lanes 7–9) in the absence (lanes 1, 4, and 7) or presence (lanes 2, 3, 5, 6, 8, and 9) of methyl donor. The results support the cross-talk between histone H4 arginine methylation and lysine acetylation. The enzymatic activity was evaluated by western blot analysis using antibodies specific for H4K5ac and for H4R3me2s. b Scatter plot of the quantification of the immunoreactive bands from three independent western blots for each of the experimental conditions described in a. One-way ANOVA with Bonferroni multiple comparison test, *p < 0.05, **p < 0.01, and ***p < 0.001. c Representative experiment showing the order of the sequential reactions. Acetylation–methylation reaction (lane 1-3): KAT7-mediated acetylation of H4 followed by symmetric postmethylation by PRMT5 in the absence (lane 1) or presence (lanes 2 and 3) of methyl donor. Ordered methylation–acetylation reaction (lanes 4–6): PRMT5-dependent symmetric premethylation of histone H4 followed by KAT7-mediated acetylation (lanes 4–6). The enzymatic activity was evaluated by western blot analysis using antibodies specific for H4K5ac and for H4R3me2s. d Scatter plot of the quantification of the immunoreactive bands from three independent western blots for each of the experimental conditions described in c. One-way ANOVA with Bonferroni multiple comparison test, *p < 0.05)