Fig. 8

Inhibitors of histone acetyltransferases rescue the effect of PRMT5 loss of activity. a Representative confocal images of OPCs cultured in differentiating conditions for 24 and 48 h with DMSO, GSK591, GSK591 + KAT inhibitors, KAT inhibitors (Butyrolactone-3, 100 μM and NU-9056, 0.2 μM) alone, and with the HDAC inhibitor Trichostatin A (TSA, 20 nM). Cells were then stained for CNP (red), MBP (white), H4K5ac (green), and DAPI (blue) (scale bar: 10 μm). b Scatter plots of the average number of CNP⁺ (24 h, upper panel) and MBP⁺ (48 h, lower panel) cell relative to total DAPI⁺ cells counted in five wells from three biological replicates. An average number of 367 cells was counted per condition and referred as percentage of the values in the DMSO treated cells. One-way ANOVA with multiple comparison test, *p < 0.05, **p < 0.01, and ***p < 0.001. c, d qRT-PCR of (c) early (Gpr17, Myt1) and (d) late (Cnp, Mbp) oligodendrocyte lineage gene transcripts measured in six independent preparations and normalized to the geo-mean of three housekeeping genes (18s, Wdr33, and Pja2) and to the internal control (DMSO) at 48 h after treatment. Values were referred to those of the internal control samples (DMSO). One-way ANOVA with Bonferroni multiple comparison test performed for each gene relative to control (*p < 0.05, **p < 0.01, and ***p < 0.001). n = 6 independent preparations