Fig. 3
Phenotype in neuronal distribution after knockdown of candidate genes during brain development. a Distribution of cells in brains electroporated with shRNAs against candidate genes at E14.5 and imaged 4 days later at E18.5. Two shRNAs against each genes were used for each genes. Brains electroporated with shRNAs targeting Cdon, Nrg3, and Itgb3 all altered neuron distribution with the accumulation of GFP+ cells in the VZ/SVZ compared to the control brain. Overexpression of these genes rescue the phenotype caused by shRNA (bottom row). b Knockdown of the excluded genes produced more limited effects. Bar = 100 μm. c Distributions of cells in the brains electroporated with shRNA targeting 25 genes from the screening. n = 3 animals for each group. *: p < 0.05, **: p < 0.01, Student’s t test. Error bars = s.d. d Distributions of cells in the brains electroporated with shRNA targeting seven of the excluded genes from the screening. n = 3 animals for each group. *: p < 0.05, Student’s t test. Error bars = s.d. e Summary of cell distribution phenotypes by shRNAs targeting the candidate and excluded genes
