Fig. 1 | Nature Communications

Fig. 1

From: miR-23b and miR-218 silencing increase Muscleblind-like expression and alleviate myotonic dystrophy phenotypes in mammalian models

Fig. 1

Validation of candidate MBNL1 and/or MBNL2 regulatory miRNAs. Logarithmic representation on base 2 (log2) of the qRT-PCR quantification of MBNL1 (a) and MBNL2 (b) expression relative to GAPDH gene in HeLa cells transfected with the indicated pCMV-MIR plasmids. c–f Relative protein expression levels of MBNL1 (c, e) and MBNL2 (d, f) in HeLa cells transfected as above. β-ACTIN was the endogenous control. In all cases, empty pCMV-MIR-GFP plasmid (VTC) was used as reference value for relative quantification, miR-37266 and miR-7 were used as positive and negative controls, respectively. GFP was used as transfection control. (n = 3). Data are mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 according to Student’s t test

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