Fig. 2 | Nature Communications

Fig. 2

From: miR-23b and miR-218 silencing increase Muscleblind-like expression and alleviate myotonic dystrophy phenotypes in mammalian models

Fig. 2

Confirmation of miRNA binding to MBNL1 and MBNL2 3′UTRs. a, e Scale representation of MBNL1 (a) and MBNL2 (e) 3′ UTRs and predicted miRNA binding sites according to miRanda and TargetScan algorithms. MBNL1 (b–d) and MBNL2 (f–h) 3′ UTR luciferase reporter assays of HeLa cells co-transfected with wild-type (b, f) or mutated (c, d, g, h) versions of 3′ UTR fused to Gaussia luciferase and miRNA plasmids (n = 4). miR-7 was used as a negative control. Wild-type (WT) reporter plasmids had the natural sequence of the miRNA binding sites, mutated (MUT) constructs lacked a candidate miRNA seed region recognition site and the perfect match (PM) versions had the miRNA binding site replaced by the full complementary sequence. **p < 0.01, ***p < 0.001 according to Student’s t test. Data are mean ± SEM

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