Fig. 2

General workflow of data acquisition and processing. a Different conditions are generated sequentially with multiple replicates (aqueous plugs) produced for each condition and collected in a tube. The whole experiment (run) is repeated multiple times and data for each run are processed separately and then combined. b Example of a plug sequence and corresponding readout. Each aqueous plug is generated by mixing the following components: cells (with orange-fluorescent dye to verify the proper mixing of all components), caspase-3 substrate and one or two compounds. Plugs of each tested condition are preceded by binary barcode plugs to encode the corresponding identification number (high concentration of blue fluorescent dye = 1, low concentration = 0, followed by an end of barcode signal)