Fig. 6

FoG and SMG levels differ between persistent and non-persistent isolates. a Candida albicans isolates from bloodstream infections were either efficiently cleared by a single course of FLC treatment (non-persistent) or persisted in the host despite multiple rounds of FLC therapy. b FoG and RAD levels for isolates SC5314, GC75, P78042, and P87 (reference isolates studied in Figs. 1–5), resistant (R, n = 1) isolate P60002³¹, the non-persistent isolates (NP, n = 7), and only the first patient isolate from each of the series of clinically persistent strains (P, n = 12). Asterisks indicate significant differences between persistent and non-persistent isolates (t test, ***P < 0.001). c Broth microdilution assays showing MIC and SMG levels at 24 and 48 h for the susceptible control strains, the non-persistent isolates as in b and for both the first and last isolates for each of the 12 clinically persistent series (S01–12). The final isolate of the S03 series of persistent strains had become FLC-resistant (MIC > 128 µg/ml, Supplementary Fig. 10a), and therefore the penultimate isolate, which remained susceptible, was used across analyses. Asterisks indicate significant differences between persistent and non-persistent isolates (t test, ***P < 0.001). For all panels isolates were tested in three or more biological replicates, error bars denote standard deviations