Fig. 1 | Nature Communications

Fig. 1

From: Intravital imaging-based analysis tools for vessel identification and assessment of concurrent dynamic vascular events

Fig. 1

Non-invasive imaging of the ear dermis vasculature. a Mice were anesthetized and immobilized to fix the ear under a water-immersion objective lens for intravital imaging. Cannulation of the tail vein allowed administration of fluorescent labeled dextrans or other carriers into the circulation. A sub-micron glass capillary was used for atraumatic intradermal injection. b Examples of the leakage from blood vessels in response to micro-injection of VEGFA164 but not PBS. Glass capillary was filled with 1 μl VEGFA164 (1 μg/μl) and Alexa633 dye (cyan); about 0.1 μl was injected. Green, 2000 kDa FITC-Dextran. Red, 70 kDa TRITC-Ficoll. Images were acquired 5 min before and 30 min after injection. Bar, 50 μm

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