Fig. 3 | Nature Communications

Fig. 3

From: Intravital imaging-based analysis tools for vessel identification and assessment of concurrent dynamic vascular events

Fig. 3

RVDM principles and verification. a RBC image is influenced by the speed of laser scanning relative to blood flow velocity and direction resulting in distorted RBC dimensions. b sXYT imaging produces distorted RBC images from which RBC dimensions Xs and Ys and RBC residence time are measured within the scan field (T), thus allowing RBC velocity quantification (see Supplementary Fig. 2 and Methods). c Comparison of RBC velocities estimated from sXYT combined with RVDM, XT, and fXYT image acquisition in the same vessels. Flow angles are indicated for each measurement. n = 3 mice with more than 20 measurements/vessel

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