Fig. 2

Ori inhibits NLRP3 inflammasome assembly by blocking NEK7–NLRP3 interaction. a Western blot analysis of cross-linked ASC in the Nonidet P-40–insoluble pellet of BMDMs stimulated with nigericin in the presence or absence of Ori. b An endogenous immunoprecipitation (IP) with NEK7 antibody or isotype antibody was performed in LPS-primed BMDMs stimulated with nigericin in the presence or absence of Ori. c An endogenous IP with ASC antibody or isotype antibody was performed in LPS-primed BMDMs stimulated with nigericin in the presence or absence of Ori. d IP and western blot analysis of NEK7–NLRP3 interaction in HEK-293T cells. e IP and western blot analysis of the interaction between purified NEK7 and NLRP3. f IP and western blot analysis of NLRP3 oligomerization in HEK-293T cells. g IP and western blot analysis of ASC-NLRP3 interaction in HEK-293T cells. Data are representative of three independent experiments