Fig. 5

Ori binds to NLRP3 via its active carbon–carbon double-bond. a Structure of Ori without carbon–carbon double-bond (R-Ori). b Cell lysates of LPS-primed BMDMs were incubated with bio-Ori (1 μM) or indicated doses of bio-R-Ori for 2 h, and then pulled down using streptavidin beads. c, d Western blot analysis (c) of cleaved IL-1β and activated caspase-1 or ELISA of IL-1β (d) in SN from LPS-primed BMDMs pretreated with Ori (2 μM) or R-Ori (2 μM) for 0.5 h and then stimulated with nigericin. Data are expressed as mean and s.e.m (n = 6) from three independent experiments (d) or are representative of three independent experiments (b, c). Statistical differences were calculated by unpaired Student’s t-test: ***P < 0.001