Fig. 3
Nuclear KMT2D expression in primary and recurrent aGCTs. a Representative stained sections from tumors with positive (GCT004) and negative (GCT017) nuclear KMT2D expression. Scale bar = 200 μm. b Density plot of per nucleus mean KMT2D staining by KMT2D mutation status. Plots were derived from measurements made from individual nuclei in KMT2D wt/trunc (n = 1,964,133 nuclei) and KMT2D wt/wt (n = 12,121,768) tumors. Dotted line indicates numerical cutoff to distinguish “negative” from “positive” nuclei (see Methods). c Relationship between mono-allelic KMT2D-truncating mutation and loss of KMT2D protein expression by tumor type. fs: frameshift mutation, trunc: truncating mutation (any class)
