Fig. 1 | Nature Communications

Fig. 1

From: TGF-β signaling alters H4K20me3 status via miR-29 and contributes to cellular senescence and cardiac aging

Fig. 1

Loss of H4K20me3 promotes cellular senescence. a Western blot for total protein of the indicated histone modifications from serially passaged MEFs. H3, H4 and α-tubulin served as loading controls. b Western blots showing expression of p16, p15, p21, Suv4-20h1 and Suv4-20h2 during MEFs senescence. β-actin served as a loading control. c, d Real-time qPCR analysis of Suv4-20h1 (c) and Suv4-20h2 (d) mRNA expression in different passages of MEFs. e, f Western blot (e) and RT-qPCR (f) for Suv4-20h in knockdown cells. g Growth curve of Suv4-20h-depleted cells. h Expression of p16, p15 and p21 in Suv4-20h knockdown cells. β-actin served as a loading control. i SA-β-gal staining in control (Ctrl) and Suv4-20h (shSuv4-20h) knockdown cells. Scale bar, 20 μm. j Immunofluorescent staining of Mki67 in control (Ctrl) and Suv4-20h depleted cells. Scale bar, 5 μm. Two-tailed unpaired Student t-tests were performed, **p < 0.01, ***p < 0.001

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