Fig. 1

Identification of DIMs and its modulators. a Systems used to induce DNA damage. b Confocal imaging of nuclei in cells under non-damaging and DNA-damaging conditions. Arrowheads, MTOC (magenta) and microtubule filaments (yellow). c, d Intranuclear microtubule filament quantitation via confocal microscopy. Veh vehicle. e Confocal imaging showing DIM emanating from an S-phase cell MTOC and differing from the mitotic spindle. f DIM length measurements. g Quantification of BIR-DSB repair. WT wild type. h, i Effect of different gene knockouts on DIM levels. Wild-type cells in (c, h) are the same. j Effect of relieving centromeric constraint on DIMs. k Effect of combining BIR-DSB induction with the relief of centromeric constraint. b, e Scale bar, 1 µm. c, d, f, g Mean ± s.d.; N = 3. *P ≤ 0.03, two-tailed unpaired t test. h–k Mean ± s.d.; N = 3. *P ≤ 0.015, two-way ANOVA Sidak’s multiple comparison test. n.s., not statistically significant. Statistical symbols in blue indicate a comparison to undamaged WT control, magenta to damaged WT control, and black compares undamaged to damaged cells for each genotype. Individual data points of undamaged (blue) and damaged (magenta) cells are shown