Fig. 2

DIMs capture/mobilize damaged DNA and promote DNA repair. a Systems used to visualize damaged DNA in live wild-type cells. b, c Time-lapse super-resolution microscopy showing the dynamics of Rad52-marked damaged DNA (yellow arrowhead) relative to DIMs. Scale bar, 1 µm. d Quantification of the proportion of DIM-positive S-phase cells with and without Rad52 foci. Mean ± s.d.; N = 3. *P = 0.0039, two-tailed unpaired t test. e Relative effect of rad9Δ or kar3Δ on the ability of DIMs to capture damaged DNA. Relative mean ± s.d.; N = 3. *P ≤ 0.0011, two-tailed unpaired t test. f Zip or control DNA sequence integration near the BIR-DSB site. g Effect of the zip code on BIR-DSB repair. Mean ± s.d.; N = 3. *P = 0.0007, two-tailed unpaired t test. h Quantification and representative images from confocal microscopy showing the effect of the zip code on DIM levels in the absence or presence of BIR-DSB induction. Mean ± s.d.; N = 5. *P ≤ 0.0067, two-way ANOVA Sidak’s multiple comparison test. Scale bar, 1 µm. Individual data points of undamaged (blue) and damaged (magenta) cells are shown