Fig. 3
Damaged DNA exhibits non-linear directional motion in the nucleus. a DIM (magenta sticks) angle (Θ) deviations of up to 0.25 rad from a straight line can cause DSB single particles (blue) to exhibit non-linear directional motion detectable by DCD, not MSD. b System used in DCD analysis of BIR-DSBs. Rad52-YFP-marked damaged DNA was tracked relative to the perinuclear Nup49-mCherry. c DCD analysis of damaged DNA mobility reveals non-linear directionality. Shown are relative angle distributions with indicated temporal coarse grainings (Δ) for a single induced BIR-DSB. Histograms are for Rad52-YFP particles monitored for 3 min using 1.5 s long steps. d DCD-derived probabilities of BIR-DSB-2 moving with up to 0.1 or 0.25 rad angle deviation from a straight line were plotted relative to MSD-derived Rc values. Error bars, DCD probability errors. e DCD-derived distributions of the velocities of damaged DNA particles for cells shown in d. f, g Comparison of DCD (f) and MSD (g) analyses for a single damaged DNA particle shown in Fig. 2b and Supplementary Movie 1. Blue and red shading, respectively, highlight either (f) DCD peaks detecting directional and anti-directional behavior or (g) time windows during which the particle is known to exhibit directional or non-directional motion along DIMs in Supplementary Movie 1
