Fig. 4
Damage increases the directional motion of DNA. a, b MAT-DSB schematic (a) and system used to visualize it relative to the nuclear periphery (b). c For a single MAT-DSB locus that is imaged under DNA damaging or non-damaging conditions, the DCD-derived probabilities of the locus moving with up to 0.1 or 0.25 rad angle (Θ) deviation from a straight line were plotted relative to MSD-derived Rc values. Error bars, DCD probability errors. d DCD-derived distributions of the velocities of the MAT-DSB locus under damaging and non-damaging conditions for the four cell types shown in c. Symbols are not shown as no difference was observed between the eight distributions. e DIM-dependent non-linear directional motion of damaged DNA in nuclear space. *Effect seen for MMS but not BIR-DSB. f Directionality and subnuclear exploration of intact and damaged DNA
